TY - JOUR

T1 - Antimicrobial susceptibility of Staphylococcus hyicus isolated from exudative epidermitis in pigs

AU - Wegener, H. C.

AU - Watts, J. L.

AU - Salmon, S. A.

AU - Yancey, R. J.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Exudative epidermitis or greasy pig syndrome is caused by the coagulase- variable staphylococcal species Staphylococcus hyicus. Treatment of this disease is problematic because of the limited number of antimicrobial agents available for this purpose. Thirteen antimicrobial agents were evaluated for their activities against 100 S. hyicus strains isolated from pigs with exudative epidermitis. Novobiocin was the most active compound tested, with an MIC for 90% of the strains tested (MIC90) of ≤0.06 μg/ml. Enrofloxacin, ampicillin, and ceftiofur were the next most active compounds, with MIC90s of 0.25, 0.5, and 1.0 μg/ml, respectively. However, 41.4% of the 99 strains tested were positive for β-lactamase production. The MIC90s of erythromycin, tetracycline, and streptomycin were >32.0 μg/ml. Initial testing with sulfadiazine-trimethoprim yielded an MIC90 of >64.0 μg/ml, but subsequent testing with thymidine phosphorylase-supplemented medium yielded an MIC90 of 0.06 μg/ml. Both lincomycin and spectinomycin were relatively inactive against the S. hyicus strains tested, with MIC90s of >64.0 and >128.0 μg/ml, respectively. However, the combination of the two compounds at ratios of 1:2 (lincomycin to spectinomycin) and 1:8 were more active, with MIC90s of 16.0 and 4.0 μg/ml, respectively. These results indicate that novobiocin and sulfadiazine-trimethoprim were the most active compounds tested against the S. hyicus strains isolated from pigs with exudative epidermitis. Furthermore, the combination of lincomycin and spectinomycin was more active than the individual compounds against the strains tested.

AB - Exudative epidermitis or greasy pig syndrome is caused by the coagulase- variable staphylococcal species Staphylococcus hyicus. Treatment of this disease is problematic because of the limited number of antimicrobial agents available for this purpose. Thirteen antimicrobial agents were evaluated for their activities against 100 S. hyicus strains isolated from pigs with exudative epidermitis. Novobiocin was the most active compound tested, with an MIC for 90% of the strains tested (MIC90) of ≤0.06 μg/ml. Enrofloxacin, ampicillin, and ceftiofur were the next most active compounds, with MIC90s of 0.25, 0.5, and 1.0 μg/ml, respectively. However, 41.4% of the 99 strains tested were positive for β-lactamase production. The MIC90s of erythromycin, tetracycline, and streptomycin were >32.0 μg/ml. Initial testing with sulfadiazine-trimethoprim yielded an MIC90 of >64.0 μg/ml, but subsequent testing with thymidine phosphorylase-supplemented medium yielded an MIC90 of 0.06 μg/ml. Both lincomycin and spectinomycin were relatively inactive against the S. hyicus strains tested, with MIC90s of >64.0 and >128.0 μg/ml, respectively. However, the combination of the two compounds at ratios of 1:2 (lincomycin to spectinomycin) and 1:8 were more active, with MIC90s of 16.0 and 4.0 μg/ml, respectively. These results indicate that novobiocin and sulfadiazine-trimethoprim were the most active compounds tested against the S. hyicus strains isolated from pigs with exudative epidermitis. Furthermore, the combination of lincomycin and spectinomycin was more active than the individual compounds against the strains tested.

UR - http://www.scopus.com/inward/record.url?scp=0028123488&partnerID=8YFLogxK

M3 - Journal article

C2 - 8195396

AN - SCOPUS:0028123488

VL - 32

SP - 793

EP - 795

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

ER -